ProDots Recombinant Human IGF-I/IGF-1 Protein Summary
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Reconstitution||For a stock solution, reconstitute at 200 μg/mL in sterile PBS, or simply roll ProDot ® directly into cell culture medium for immediate use.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 degreesC as supplied. • 1 month at 2-8 degreesC under sterile conditions after reconstitution. • 3 months at -20 to -80 degreesC under sterile conditions after reconstitution.|
ProDots® Recombinant Human IGF-I/IGF-1 stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3-1.5 ng/mL.
1 μg/lane of ProDots® Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 7 kDa.
Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity. The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.
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