IGF-1 (IGF-I, insulin-like growth factor-1 or somatomedin C) is a secreted factor that circulates in complex with IGFBP-3 or IGFBP-5 with ALS. It signals through dimeric IGF1R, dimeric Insulin R, or a heterodimer of the two receptors.
ProDots Recombinant Human IGF-I/IGF-1 Protein
R&D Systems | Catalog # PRD291
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD291 has been discontinued.
View all IGF-I/IGF-1 products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
E. coli
Accession Number
Applications
Bioactivity
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Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
E. coli-derived human IGF-I/IGF-1 protein
Gly49-Ala118
Gly49-Ala118
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly49
Predicted Molecular Mass
7.6 kDa
SDS-PAGE
7 kDa, reducing conditions
Activity
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 0.3-1.5 ng/mL.
The ED50 for this effect is 0.3-1.5 ng/mL.
Scientific Data Images for ProDots Recombinant Human IGF-I/IGF-1 Protein
ProDots Recombinant Human IGF-I/IGF-1 Protein Bioactivity
ProDots®Recombinant Human IGF-I/IGF-1 stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3-1.5 ng/mL.
ProDots Recombinant Human IGF-I/IGF-1 Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 7 kDa.Formulation, Preparation, and Storage
PRD291
| Reconstitution | For a stock solution, reconstitute at 200 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: IGF-I/IGF-1
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
UniProt
Additional IGF-I/IGF-1 Products
Product Documents for ProDots Recombinant Human IGF-I/IGF-1 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human IGF-I/IGF-1 Protein
726248.1
For research use only
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