Q: Does IL-1 beta induce NO in smooth muscle?

A: Yes it does, based on the literature.

ProDots Recombinant Human IL-1 beta/IL-1F2 Protein

R&D Systems | Catalog # PRD201

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Product Documents

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD201 has been discontinued. View all IL-1 beta/IL-1F2 products.

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Features and Benefits

Rolls out of bottle for easy cell culture medium preparation
Eliminates time spent aliquoting
One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Source

E. coli

Accession Number

NP_000567

Applications

Bioactivity

View all IL-1 beta/IL-1F2 Proteins and Enzymes »

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?

ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.

Product Specifications

Source

E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117 - Ser269

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala117 & Pro118

Predicted Molecular Mass

17 kDa

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED₅₀ for this effect is <12 pg/mL.

Scientific Data Images for ProDots Recombinant Human IL-1 beta/IL-1F2 Protein

ProDots Recombinant Human IL-1 beta/IL-1F2 Protein Bioactivity

ProDots^®Recombinant Human IL-1 beta /IL-1F2 stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED₅₀ for this effect is <12 pg/mL.

ProDots Recombinant Human IL-1 beta/IL-1F2 Protein SDS-PAGE

1 μg/lane of ProDots^®Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 17 kDa.

Formulation, Preparation, and Storage

PRD201

Reconstitution	For a stock solution, reconstitute at 25 μg/mL in sterile PBS, or simply roll ProDot^® directly into cell culture medium for immediate use.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.