Proenkephalin Antibody

Novus Biologicals | Catalog # NBP1-20963

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Proenkephalin Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YKDSSKQDESH corresponding to internal region according to Uniprot Mouse NP_001002927.1.

Epitope

C terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Proenkephalin Antibody

Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963]

Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963]

Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963]

Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963]

Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Mouse Stria terminalis. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Applications for Proenkephalin Antibody

Application
Recommended Usage

Immunohistochemistry

0.02 - 0.05 ug/ml

Immunohistochemistry-Frozen

0.02-0.05 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 40 kDa band in rat brain lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8 kDa band according to mouse NP_001002927.1 and 30.9 kDa band according to rat NP_058835.1. The 40 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-Fr: Rat brain (striatum) shows staining of dense enkephalinergic axon plexus. Mouse brain (stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proenkephalin

Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, including pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.

Alternate Names

enkephalin A, preproenkephalin, proenkephalin, proenkephalin-A

Entrez Gene IDs

110182 (Mouse); 29237 (Rat)

Gene Symbol

PENK

UniProt

NP_001002927.1 (Mouse)

Additional Proenkephalin Products

Product Documents for Proenkephalin Antibody

Certificate of Analysis

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Product Specific Notices for Proenkephalin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Proenkephalin Antibody

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