Profilin 1 Antibody (CL3524) - BSA Free

Novus Biologicals | Catalog # NBP2-59778

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL3524

Format

BSA Free
View all Profilin 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P07737, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Rat (87%), Mouse (87%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Profilin 1 Antibody (CL3524) - BSA Free

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Profilin 1 Antibody (5216) [NBP2-59778] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PFN1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human testis shows weak immunoreactivity in basal cells of the seminiferous ducts.
Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (5216) [NBP2-59778] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.
Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human fallopian tube shows cytoplasmic immunoreactivity in epithelial cells.
Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human tonsil shows moderate to strong immunoreactivity in lymphoid cells.
Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human liver shows strong immunoreactivity in circulating lymphoid cells.
Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human kidney shows strong membranous combined with moderate cytoplasmic immunoreactivity in renal tubules.
Knockout Validated: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Lysates of HAP1 WT and PFN1 KO were prepared, and 50 ug of protein were processed for immunoblot with Profilin 1 antibody (NBP2-59778). The Ponceau stained transfer of the blot is shown. Antibody dilution used: NBP2-59778 at 1/1000. Predicted band size: 15 kDa. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Profilin 1 Antibody (CL3524) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Profilin 1

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Profilin 1 Antibody (CL3524) - BSA Free

Certificate of Analysis

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Product Specific Notices for Profilin 1 Antibody (CL3524) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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