Properdin Antibody (062-27.8.1 (10-18)) - BSA Free

Novus Biologicals | Catalog # NB100-64749

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 062-27.8.1 (10-18)

Format

BSA Free
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Product Specifications

Immunogen

Native purified factor P from human serum

Specificity

NB100-64749 recognizes human factor P, a 53 kDa glycosylated protein present in blood serum. Factor P binds to microbial surfaces and binds and stabilizes the complement component 3- and complement component 5-convertase enzyme complexes. C3b then acts as an opsonin or initiates the complement pathway again creating a positive feedback loop which acts only on the bacterial cell, leaving the host cells unharmed. Deficiency of factor P makes the host more susceptible to bacterial infections, particularly to Neisseria species and meningococcal infections.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Properdin Antibody (062-27.8.1 (10-18)) - BSA Free

Western Blot: Properdin Antibody (10-18)BSA Free [NB100-64749]

Western Blot: Properdin Antibody (10-18)BSA Free [NB100-64749]

Western Blot: Properdin Antibody (10-18) [NB100-64749] - Human serum was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. In lane 1, Bio-Rad Precision Plus molecular weight marker was run. In lane 2, Mouse anti Human Factor P was used as the primary antibody. HRP conjugated Goat anti Mouse IgG (H/L) was used as the secondary antibody. As a control, HRP conjugated Goat anti Mouse IgG (H/L) was run in lane 3. Visualization was carried out using the Bio-Rad ChemiDoc Imaging System with automatic exposure.

Applications for Properdin Antibody (062-27.8.1 (10-18)) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Reviewed Applications

Read 1 review rated 1 using NB100-64749 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Borate buffered saline

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Properdin

Properdin in plasma is a mixture of cyclic dimers, trimers and tetramers. The molecular weight of the glycosylated monomer is 53 kDa. Properdin is a regulator protein of the alternative complement pathway. It stabilizes the C3 convertase excreting its effect by binding to C3b in the C3bBb complex and thereby inhibiting cleavage of C3b by factor I and increasing the affinity for factor B. Serum concentration is approximately 25 ug/mL.

Alternate Names

CFP, Complement Factor P, PFC

Entrez Gene IDs

5199 (Human)

Gene Symbol

CFP

UniProt

P27918

Additional Properdin Products

Product Documents for Properdin Antibody (062-27.8.1 (10-18)) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Properdin Antibody (062-27.8.1 (10-18)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Complement Pathway

Customer Reviews for Properdin Antibody (062-27.8.1 (10-18)) - BSA Free (1)

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  • Properdin Antibody (10-18)
    Name: Silvia Torchio
    Application: Western Blot
    Sample Tested: HepG2, iPS2 human induced pluripotent stem cells and IPS2 cells differentiated to endocrine progenitor cells
    Species: Human
    Verified Customer | Posted 12/29/2020
    Blot of HepG2 and iPSCs derivatives at various stages of pancreatic differentiation. Antibody diluted 1:500 in milk 5% in TBST or 1:1000 in BSA 5% in TBST, incubated overnight at 4°C. Markers visible on both side lanes are: 55kDa, 35 kDa, 15kDa.
    Properdin Antibody (062-27.8.1 (10-18)) - BSA Free NB100-64749

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