Protease Activity Assays & Reagents

Fluorokine® E Matrix Metalloproteinase Activity Assays

Assay Principle

View Full Assay Principle

R&D Systems Fluorokine E Activity Assays offer a consistent method for quantifying pro and active MMPs side-by-side on the same plate. Monoclonal antibodies capture both pro and mature MMPs, and a fluorogenic peptide substrate detects the levels of endogenous active MMP. MMPs can be activated in the sample using the provided APMA and the levels of the pro form deduced.

Kit Contents

  • 96-well Microplate
  • Fluorogenic Substrate
  • MMP Standard
  • Assay, Calibrator, and Reagent Diluents
  • Wash Buffer
  • p-Aminophenylmercuric Acetate (APMA)

Caspase Activity Assays

A range of plate-based assays are available to measure Caspase activity. These kits are Caspase-specific and utilize a peptide coupled to a colorimetric or fluorometric reporter system to assess activity in cell lysates.

Kit Contents

  • 96-well Microplate
  • Colorimetric or Fluorometric Reporter Substrate
  • Extraction, Dilution, and Reaction Buffers
  • Dithiothreitol

Fluorogenic Peptide Substrates

In response to proteolytic cleavage, Fluorogenic Peptide Substrates emit a signal that can be measured using a fluorescence plate reader. Many substrates are available including those suitable for assessing the activity of Matrix Metalloproteinases (MMPs), Cathepsins, Kallikreins, Coagulation Factors, and more.

Caspase and Serine Protease Inhibitors

Cell permeable fluoromethyl ketone (FMK)-derivatized peptides act as effective irreversible Caspase inhibitors with no cytotoxic effects and, therefore, are useful tools for studying Caspase activity. Caspase-specific and Pan-specific inhibitors are available as well as sampler packs. AEBSF, a cell permeable general serine protease inhibitor, is also available.