Protease Inhibitor 15 Antibody

Name: Protease Inhibitor 15 Antibody
Brand: Novus Biologicals
SKU: NBP2-11581
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-11581

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-RTGRYRSILQLVKP corresponding to internal region according to NP_056970.1.

Epitope

CRTGRYRSILQLVKP

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Protease Inhibitor 15 Antibody

Western Blot: Protease Inhibitor 15 Antibody [NBP2-11581]

Western Blot: Protease Inhibitor 15 Antibody [NBP2-11581] - HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP2-11581]

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP2-11581] - Human Skeletal Muscle. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Protease Inhibitor 15 Antibody

Application

Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:1000

Western Blot

0.3 - 1 ug/mL

Application Notes

WB: Approx. 33 kDa band observed in HeLa lysates (calculated MW of 29.1 kDa band according to NP_056970.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protease Inhibitor 15

Protease Inhibitor 15 encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system.

Alternate Names

25 kDa trypsin inhibitor, CRISP8, CRISP-8, Cysteine-rich secretory protein 8, P24TI, p25TI, P25TIDKFZp686F0366, peptidase inhibitor 15, PI-15, protease inhibitor 15, sugarCrisp

Entrez Gene IDs

51050 (Human); 94227 (Mouse); 301489 (Rat)

Gene Symbol

PI15

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Product Documents for Protease Inhibitor 15 Antibody

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Certificate of Analysis

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Product Specific Notices for Protease Inhibitor 15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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