Proteasome 20S beta 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33516

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Proteasome 20S beta 3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 20S beta 3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Proteasome 20S beta 3 Antibody [NBP2-33516]

Immunocytochemistry/ Immunofluorescence: Proteasome 20S beta 3 Antibody [NBP2-33516]

Immunocytochemistry/Immunofluorescence: Proteasome 20S beta 3 Antibody [NBP2-33516] - Staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: Proteasome 20S beta 3 Antibody [NBP2-33516]

Immunohistochemistry-Paraffin: Proteasome 20S beta 3 Antibody [NBP2-33516]

Immunohistochemistry-Paraffin: Proteasome 20S beta 3 Antibody [NBP2-33516] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.

Applications for Proteasome 20S beta 3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome 20S beta 3

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12.

Long Name

Proteasome subunit beta type-3

Alternate Names

Proteasome chain 13, PSMB3

Gene Symbol

PSMB3

UniProt

P49720 (Human)

Additional Proteasome 20S beta 3 Products

Product Documents for Proteasome 20S beta 3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Proteasome 20S beta 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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