Proteasome 20S beta2 Antibody (M1) - Azide and BSA Free

Novus Biologicals | Catalog # H00005690-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M1

Format

Azide and BSA Free
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Product Specifications

Immunogen

PSMB2 (AAH00268, 1 a.a. ~ 201 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS

Specificity

PSMB2 - proteasome (prosome, macropain) subunit, beta type, 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Proteasome 20S beta2 Antibody (M1) - Azide and BSA Free

Immunohistochemistry-Paraffin: Proteasome 20S beta2 Antibody (M1) [H00005690-M02]

Immunohistochemistry-Paraffin: Proteasome 20S beta2 Antibody (M1) [H00005690-M02]

Immunohistochemistry-Paraffin: Proteasome 20S beta2 Antibody (M1) [H00005690-M02] - Analysis of monoclonal antibody to PSMB2 on formalin-fixed paraffin-embedded human endometrium.Antibody concentration 3 ug/ml.
Sandwich ELISA: Proteasome 20S beta2 Antibody (M1) [H00005690-M02] - Detection limit for recombinant GST tagged PSMB2 is approximately 0.03ng/ml as a capture antibody.

Applications for Proteasome 20S beta2 Antibody (M1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. It has been used for IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Proteasome 20S beta2

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.

Long Name

Proteasome subunit beta type-2

Alternate Names

PSMB2

Entrez Gene IDs

5690 (Human)

Gene Symbol

PSMB2

OMIM

602175 (Human)

Additional Proteasome 20S beta2 Products

Product Documents for Proteasome 20S beta2 Antibody (M1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Proteasome 20S beta2 Antibody (M1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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