Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NB100-1921
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Primate
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Purified full length P4hb isolated from rat liver.
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free
Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NB100-1921] -
Normal human brain.
Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NB100-1921] -
ICC/IF Confocal analysis of 3T3 cells using P4HB antibody (NB100-1921, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NB100-1921] -
IHC analysis of a formalin fixed paraffin-embedded (FFPE) human brain using 1:250 conc. of P4HB antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of P4HB in neurons was observed. Staining was performed by Histowiz.
Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NB100-1921] -
P4HB staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).Applications for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:5000-1:20000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
1-3 ug
Western Blot
1:10000-1:20000
Application Notes
This P4hb antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.
Reviewed Applications
Read 2 reviews rated 5 using NB100-1921 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Protein Disulfide Isomerase/P4HB
Long Name
Proline 4-Hydroxylase, Beta Polypeptide
Alternate Names
DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp
Gene Symbol
P4HB
Additional Protein Disulfide Isomerase/P4HB Products
Product Documents for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free
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Customer Reviews for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free (2)
5 out of 5
2 Customer Ratings
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Customer Images
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Application: Western BlotSample Tested: Rat brainSpecies: RatVerified Customer | Posted 03/06/2015Excellent antibody. works at 1/10000 and could have diluted further the sample size is a great value for the money. Kudos
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Application: ImmunocytochemistrySample Tested:Species: MouseVerified Customer | Posted 06/16/2014IF Confocal analysis of 3T3 cells using PDI antibody (NB100-1921, 1:5).
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Protein Disulfide Isomerase/P4HB Antibody - Azide and BSA Free
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Q: What permeabilization method was used to test this antibody?
A: For our protocol, our lab uses 0.1-0.5% Tween-20 in PBS or TBS for permeabilization.
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