Protein Phosphatase 1 beta Antibody
Novus Biologicals | Catalog # NB110-40550
![Western Blot: Protein Phosphatase 1 beta Antibody [NB110-40550]](https://resources.rndsystems.com/images/products/Protein-Phosphatase-1-beta-Antibody-Western-Blot-NB110-40550-img0002.jpg "Western Blot: Protein Phosphatase 1 beta Antibody [NB110-40550]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Mouse (100%), Porcine (100%), Primate (100%), Rabbit (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 280 and the C-terminus (residue 327) of human Protein Phosphatase 1, Catalytic Subunit, Beta Isoform using the numbering given in entry NP_996759.1 (GeneID 5500).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Protein Phosphatase 1 beta Antibody
Western Blot: Protein Phosphatase 1 beta Antibody [NB110-40550]
Western Blot: Protein Phosphatase 1 beta Antibody [NB110-40550] - Detection of Human PPP1CB on HeLa whole cell lysate using NB110-40550. PPP1CC was immunoprecipitated by rabbit anti-PPP1CC antibody NB110-40551.![Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NB110-40550]](https://resources.rndsystems.com/images/products/Protein-Phosphatase-1-beta-Antibody-Immunohistochemistry-Paraffin-NB110-40550-img0004.jpg "Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NB110-40550]")
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NB110-40550]
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody [NB110-40550] - Human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP1CB used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for Protein Phosphatase 1 beta Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:250
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Protein Phosphatase 1 beta
Alternate Names
EC 3.1.3.16, EC 3.1.3.53, MGC3672, PP1beta, PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform, PPP1CD, protein phosphatase 1, catalytic subunit, beta isoform, protein phosphatase 1, catalytic subunit, beta isozyme, protein phosphatase 1-beta, protein phosphatase 1-delta, serine/threonine protein phosphatase PP1-beta catalytic subunit, serine/threonine-protein phosphatase PP1-beta catalytic subunit
Entrez Gene IDs
5500 (Human)
Gene Symbol
PPP1CB
UniProt
Additional Protein Phosphatase 1 beta Products
Product Documents for Protein Phosphatase 1 beta Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Protein Phosphatase 1 beta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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