Proteome Profiler Human Protease/Protease Inhibitor Array

Contains 4 membranes-2 spotted in duplicate with antibodies against 35 different proteases and 2 spotted in duplicate with antibodies against 32 different protease inhibitors
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R&D Systems Proteome Profiler Antibody Array Kits
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Product Details
Citations (8)

Proteome Profiler Human Protease/Protease Inhibitor Array Summary

Kit Summary

To simultaneously detect the relative changes of 32 protease inhibiting analytes in a single sample. No specialized equipment is necessary.


General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, serum, plasma, urine, saliva, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.


Kit Contents
  • 4 Array Membranes (2 of Proteases and 2 Protease Inhibitors)
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail, Human Protease Array
  • Detection Antibody Cocktail, Human Protease Inhibitor Array
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.



Simultaneously detect the levels of these protease inhibitor proteins in a single sample.
APP Lipocalin-1 Serpin F1/PEDF
Cystatin A Lipocalin-2/NGAL Testican 1/SPOCK1
Cystatin B RECK Testican 2/SPOCK2
Cystatin C Serpin A5 TFPI
Cystatin E/M Serpin A8/AGT TFPI-2
EMMPRIN Serpin A9/Centerin TIMP-1
Fetuin B Serpin A12 TIMP-2
HAI-1 Serpin B5/Maspin TIMP-3
HAI-2 Serpin B6 TIMP-4
HE4/WFDC2 Serpin B8 Trappin-2/Elafin
Latexin Serpin E1/PAI-1  


Simultaneously detect the levels of these proteases in a single sample.
ADAM8 Cathepsin X/Z/P MMP-7
ADAMTS1 Kallikrein 3/PSA MMP-9
ADAMTS13 Kallikrein 5 MMP-10
Cathepsin A Kallikrein 6 MMP-12
Cathepsin B Kallikrein 7 MMP-13
Cathepsin C/DPPI Kallikrein 10 Neprilysin/CD10
Cathepsin D Kallikrein 11 Presenilin-1
Cathepsin E Kallikrein 13 Proprotein Convertase 9
Cathepsin L MMP-1 Proteinase 3
Cathepsin S MMP-2 uPA/Urokinase
Cathepsin V MMP-3  


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Citations for Proteome Profiler Human Protease/Protease Inhibitor Array

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. C9orf72-ALS human iPSC microglia are pro-inflammatory and toxic to co-cultured motor neurons via MMP9
    Authors: Vahsen, BF;Nalluru, S;Morgan, GR;Farrimond, L;Carroll, E;Xu, Y;Cramb, KML;Amein, B;Scaber, J;Katsikoudi, A;Candalija, A;Carcolé, M;Dafinca, R;Isaacs, AM;Wade-Martins, R;Gray, E;Turner, MR;Cowley, SA;Talbot, K;
    Nature communications  2023-09-22
  2. Copper promotes cardiac functional recovery via suppressing the transformation of fibroblasts to myofibroblasts in ischemia-infarcted monkey hearts
    Authors: Y Xiao, Q Feng, L Huang, X Meng, P Han, W Zhang, YJ Kang
    The Journal of nutritional biochemistry, 2022-10-12;0(0):109180.  2022-10-12
  3. ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9
    Authors: L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch
    International Journal of Molecular Sciences, 2022-02-10;23(4):.  2022-02-10
  4. Low RECK Expression Is Part of the Cervical Carcinogenesis Mechanisms
    Authors: S Herbster, M Trombetta-, PT de Souza-S, A Paladino, CRF Silveira, MC Sogayar, LL Villa, AP Lepique, E Boccardo
    Cancers, 2021-05-06;13(9):.  2021-05-06
  5. A heterometallic ruthenium-gold complex displays antiproliferative, antimigratory, and antiangiogenic properties and inhibits metastasis and angiogenesis-associated proteases in renal cancer
    Authors: BT Elie, Y Pechenyy, F Uddin, M Contel
    J. Biol. Inorg. Chem., 2018-03-05;0(0):.  2018-03-05
  6. KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes
    Authors: T Nargis, K Kumar, AR Ghosh, A Sharma, D Rudra, D Sen, S Chakrabart, S Mukhopadhy, D Ganguly, P Chakrabart
    Mol Metab, 2017-09-27;6(11):1529-1539.  2017-09-27
  7. Proteases Revisited: Roles and Therapeutic Implications in Fibrosis
    Authors: J Kryczka, J Boncela
    Mediators Inflamm., 2017-05-31;2017(0):2570154.  2017-05-31
  8. Use of ethanol extracts of Terminalia chebula to prevent periodontal disease induced by dental plaque bacteria
    Authors: J Lee, YH Nho, SK Yun, YS Hwang
    BMC Complement Altern Med, 2017-02-16;17(1):113.  2017-02-16


  1. Does the Human Protease/Protease Inhibitor Array detect proteases/protease inhibitors complexes? 

    • This Array is validated for the detection of free proteases and protease inhibitors. The detection of protease- and protease inhibitor complexes has not been evaluated.

  2. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

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