Proteome Profiler Human Protease/Protease Inhibitor Array
Proteome Profiler Human Protease/Protease Inhibitor Array Summary
To simultaneously detect the relative changes of 32 protease inhibiting analytes in a single sample. No specialized equipment is necessary.
- Troubleshooting Guide
- Assays for analytes represented in the Human Protease/Protease Inhibitor Array Kit
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, serum, plasma, urine, saliva, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes (2 of Proteases and 2 Protease Inhibitors)
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail, Human Protease Array
- Detection Antibody Cocktail, Human Protease Inhibitor Array
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
|Simultaneously detect the levels of these protease inhibitor proteins in a single sample.|
|Cystatin A||Lipocalin-2/NGAL||Testican 1/SPOCK1|
|Cystatin B||RECK||Testican 2/SPOCK2|
|Cystatin C||Serpin A5||TFPI|
|Cystatin E/M||Serpin A8/AGT||TFPI-2|
|Fetuin B||Serpin A12||TIMP-2|
|Simultaneously detect the levels of these proteases in a single sample.|
|Cathepsin A||Kallikrein 6||MMP-12|
|Cathepsin B||Kallikrein 7||MMP-13|
|Cathepsin C/DPPI||Kallikrein 10||Neprilysin/CD10|
|Cathepsin D||Kallikrein 11||Presenilin-1|
|Cathepsin E||Kallikrein 13||Proprotein Convertase 9|
|Cathepsin L||MMP-1||Proteinase 3|
Citations for Proteome Profiler Human Protease/Protease Inhibitor Array
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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A heterometallic ruthenium-gold complex displays antiproliferative, antimigratory, and antiangiogenic properties and inhibits metastasis and angiogenesis-associated proteases in renal cancer
Authors: BT Elie, Y Pechenyy, F Uddin, M Contel
J. Biol. Inorg. Chem., 2018;0(0):. 2018
KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes
Authors: T Nargis, K Kumar, AR Ghosh, A Sharma, D Rudra, D Sen, S Chakrabart, S Mukhopadhy, D Ganguly, P Chakrabart
Mol Metab, 2017;6(11):1529-1539. 2017
Proteases Revisited: Roles and Therapeutic Implications in Fibrosis
Authors: J Kryczka, J Boncela
Mediators Inflamm., 2017;2017(0):2570154. 2017
Use of ethanol extracts of Terminalia chebula to prevent periodontal disease induced by dental plaque bacteria
Authors: J Lee, YH Nho, SK Yun, YS Hwang
BMC Complement Altern Med, 2017;17(1):113. 2017
Does ARY025 detect free protease/protease inhibitor or protease/protease inhibitor complexes?
We have confirmed ARY025 reacts with free protease and protease inhibitor. We have not evaluated protease complexes and protease inhibitor complexes in this array.
Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?
The proteinase inhibitors are recommended for cell and tissue lysates only. You do not need them for cell culture supernates.
Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
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