Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, serum, plasma, urine, saliva, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
4 Array Membranes (2 of Proteases and 2 Protease Inhibitors)
Detection Antibody Cocktail, Human Protease Array
Detection Antibody Cocktail, Human Protease Inhibitor Array
Chemiluminescent Detection Reagents
Transparency Overlay Template
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Simultaneously detect the levels of these protease inhibitor proteins in a single sample.
Simultaneously detect the levels of these proteases in a single sample.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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