Proteome Profiler Human Protease/Protease Inhibitor Array
Proteome Profiler Human Protease/Protease Inhibitor Array Summary
To simultaneously detect the relative changes of 32 protease inhibiting analytes in a single sample. No specialized equipment is necessary.
- Troubleshooting Guide
- Assays for analytes represented in the Human Protease/Protease Inhibitor Array Kit
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, serum, plasma, urine, saliva, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes (2 of Proteases and 2 Protease Inhibitors)
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail, Human Protease Array
- Detection Antibody Cocktail, Human Protease Inhibitor Array
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
|Simultaneously detect the levels of these protease inhibitor proteins in a single sample.|
|Cystatin A||Lipocalin-2/NGAL||Testican 1/SPOCK1|
|Cystatin B||RECK||Testican 2/SPOCK2|
|Cystatin C||Serpin A5||TFPI|
|Cystatin E/M||Serpin A8/AGT||TFPI-2|
|Fetuin B||Serpin A12||TIMP-2|
|Simultaneously detect the levels of these proteases in a single sample.|
|Cathepsin A||Kallikrein 6||MMP-12|
|Cathepsin B||Kallikrein 7||MMP-13|
|Cathepsin C/DPPI||Kallikrein 10||Neprilysin/CD10|
|Cathepsin D||Kallikrein 11||Presenilin-1|
|Cathepsin E||Kallikrein 13||Proprotein Convertase 9|
|Cathepsin L||MMP-1||Proteinase 3|
Preparation and Storage
No product specific FAQs exist for this product, however you mayView all Proteome Profiler Antibody Array FAQs
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