Prothrombin Antibody (9A1) - BSA Free

Novus Biologicals | Catalog # NBP3-26594

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9A1 expressed in HEK293

Format

BSA Free
View all Prothrombin Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Prothrombin [UniProt P00734]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Prothrombin Antibody (9A1) - BSA Free

Prothrombin Antibody (9A1)

Immunohistochemistry: Prothrombin Antibody (9A1) [NBP3-26594] -

Immunohistochemistry: Prothrombin Antibody (9A1) [NBP3-26594] - Image of Prothrombin Antibody (9A1) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Prothrombin Antibody (9A1) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Prothrombin

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.

Alternate Names

Coagulation factor II, coagulation factor II (thrombin), EC 3.4.21, EC 3.4.21.5, prothrombin, prothrombin B-chain, PT, serine protease

Gene Symbol

F2

Additional Prothrombin Products

Product Documents for Prothrombin Antibody (9A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Prothrombin Antibody (9A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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