PRPF31 Antibody

Novus Biologicals | Catalog # NB100-55409

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PRPF31 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KELGNSLDKCKNNEN corresponding to internal region according to NP_056444.3.

Epitope

KELGNSLDKCKNNEN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PRPF31 Antibody

Western Blot: PRPF31 Antibody [NB100-55409]

Western Blot: PRPF31 Antibody [NB100-55409]

Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of A431 (A), HeLa (B) and HEK293 (C) nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PRPF31 Antibody [NB100-55409]

Immunocytochemistry/ Immunofluorescence: PRPF31 Antibody [NB100-55409]

Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: PRPF31 Antibody [NB100-55409]

Flow Cytometry: PRPF31 Antibody [NB100-55409]

Flow Cytometry: PRPF31 Antibody [NB100-55409] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: PRPF31 Antibody [NB100-55409]

Western Blot: PRPF31 Antibody [NB100-55409]

Western Blot: PRPF31 Antibody [NB100-55409] - Staining (1ug/ml) of NIH3T3 nuclear cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PRPF31 Antibody [NB100-55409]

Immunocytochemistry/ Immunofluorescence: PRPF31 Antibody [NB100-55409]

Immunocytochemistry/Immunofluorescence: PRPF31 Antibody [NB100-55409] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for PRPF31 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in A431, HeLa, HEK293 and NIH3T3 lysates (calculated MW of 55.4 kDa band according to NP_056444.3).

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRPF31

Retinitis pigmentosa (RP) is a clinically and genetically heterogeneous group of retinal dystrophies characterized by a progressive degeneration of photoreceptors, eventually resulting in severe visual impairment.

Alternate Names

DKFZp566J153, hPrp31, NY-BR-99, Pre-mRNA-processing factor 31, Protein 61K, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), PRP31 pre-mRNA processing factor 31 homolog (yeast), PRP31RP11, Serologically defined breast cancer antigen NY-BR-99, U4/U6 small nuclear ribonucleoprotein Prp31, U4/U6 snRNP 61 kDa protein

Entrez Gene IDs

26121 (Human); 68988 (Mouse)

Gene Symbol

PRPF31

UniProt

NP_056444.2 (Human)

Additional PRPF31 Products

Product Documents for PRPF31 Antibody

Certificate of Analysis

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Product Specific Notices for PRPF31 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PRPF31 Antibody

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Protocols

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FAQs

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