PRR7 Antibody (TRAP3/10) - BSA Free

Novus Biologicals | Catalog # NBP1-30424

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # TRAP3/10

Format

BSA Free
View all PRR7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)

Reactivity Notes

Cross reacts with Human, Mouse and Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRR7 Antibody (TRAP3/10) - BSA Free

Western Blot: PRR7 Antibody (TRAP3/10)BSA Free [NBP1-30424]

Western Blot: PRR7 Antibody (TRAP3/10)BSA Free [NBP1-30424]

Western Blot: PRR7 Antibody (TRAP3/10) [NBP1-30424] - Detection of PRR7 / TRAP3 in murine brain lysate by Western blotting using the monoclonal antibody TRAP3/10.
Immunocytochemistry/ Immunofluorescence: PRR7 Antibody (TRAP3/10) - BSA Free [NBP1-30424]

Immunocytochemistry/ Immunofluorescence: PRR7 Antibody (TRAP3/10) - BSA Free [NBP1-30424]

Immunocytochemistry/Immunofluorescence: PRR7 Antibody (TRAP3/10) [NBP1-30424] - Staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).

Applications for PRR7 Antibody (TRAP3/10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

1 ug/ml
Application Notes
Immunocytochemistry: Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRR7

PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.

Alternate Names

MGC10772, proline rich 7 (synaptic), proline-rich protein 7, Synaptic proline-rich membrane protein

Gene Symbol

PRR7

Additional PRR7 Products

Product Documents for PRR7 Antibody (TRAP3/10) - BSA Free

Certificate of Analysis

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Product Specific Notices for PRR7 Antibody (TRAP3/10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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