PSD93 Antibody

Novus Biologicals | Catalog # NB300-546

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PSD93 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues N(352) K L C D K P A S P R H Y S P(366) of human PSD-93.

Localization

Cell Membrane and Cytoplasmic

Marker

post-Synaptic Marker

Specificity

Detects Post Synaptic Density 93 (PSD 93) from rat tissues. This does not detect other synapse-associated protein family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSD93 Antibody

Western Blot: PSD93 Antibody [NB300-546]

Western Blot: PSD93 Antibody [NB300-546]

Western Blot: PSD93 Antibody [NB300-546] - Western blot analysis of membrane-enriched extracts (30 ug lysate) from SHY-SY5Y (Lane 1), and Neuro-2a (Lane 2) cell lines. PSD93 antibody was used at a concentration of 3 ug/mL and the signal was developed using Goat anti-Rabbit IgG (H+L) secondary antibody (HRP conjugated) through chemiluminescence method. Approximately 115 kDa band corresponding to PSD93 was observed in both samples. The membrane was further processed for loading control blot (Tubulin) to confirm an equal loading of samples in both lanes.
Immunocytochemistry/ Immunofluorescence: PSD93 Antibody [NB300-546]

Immunocytochemistry/ Immunofluorescence: PSD93 Antibody [NB300-546]

Immunocytochemistry/Immunofluorescence: PSD93 Antibody [NB300-546] - Immunolocalization of at cerebellum.
Western Blot: PSD93 Antibody [NB300-546]

Western Blot: PSD93 Antibody [NB300-546]

Western Blot: PSD93 Antibody [NB300-546] - Analysis of PSD-93 on rat brain extract.

Applications for PSD93 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

5 ug/ml

Western Blot

3 ug/ml
Application Notes
WB: Detects an approx. 110 kDa protein representing PSD 93 in rat brain extract.Immunohistochemical staining in rat cerebellum with yields a pattern consistent with post-synaptic localization.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSD93

Post Synaptic Density 93 (PSD 93), also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD 93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD 93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity.PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Alternate Names

Chapsyn-110, discs, large homolog 2 (Drosophila), discs, large homolog 2, chapsyn-110, discs, large homolog 2, chapsyn-110 (Drosophila), disks large homolog 2, DKFZp781D1854, DKFZp781E0954, FLJ37266, MGC131811, Postsynaptic density protein PSD-93, PSD-93, PSD93,110kDa

Entrez Gene IDs

1740 (Human); 64053 (Rat)

Gene Symbol

DLG2

Additional PSD93 Products

Product Documents for PSD93 Antibody

Certificate of Analysis

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Product Specific Notices for PSD93 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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