PSMA1 Antibody (1D9-1C7) - Azide and BSA Free

Novus Biologicals | Catalog # H00005682-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D9-1C7

Format

Azide and BSA Free
View all PSMA1 Primary Antibodies »

Product Specifications

Immunogen

PSMA1 (AAH02577, 1 a.a. ~ 263 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEPAEKADEPMEH

Specificity

PSMA1 - proteasome (prosome, macropain) subunit, alpha type, 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PSMA1 Antibody (1D9-1C7) - Azide and BSA Free

Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01]

Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01]

Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Analysis of PSMA1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody (1D9-1C7) [H00005682-M01]

Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody (1D9-1C7) [H00005682-M01]

Immunocytochemistry/Immunofluorescence: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of monoclonal antibody to PSMA1 on HeLa cell. Antibody concentration 60 ug/ml.
Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01]

Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01]

Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of PSMA1 over-expressed 293 cell line, cotransfected with PSMA1 Validated Chimera RNAi ( Cat # H00005682-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMA1 monoclonal antibody (M01), clone 1D9-1C7 (Cat # H00005682-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01]

Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01]

Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody (M01), clone 1D9-1C7.Lane 1: PSMA1 transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.

Applications for PSMA1 Antibody (1D9-1C7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSMA1

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Long Name

Proteasome (Prosome, Macropain) Subunit, alpha Type, 1

Alternate Names

Macropain Subunit C2, P30-33K, PROS30, Proteasome Component C2, Proteasome nu Chain, PSC2

Entrez Gene IDs

5682 (Human)

Gene Symbol

PSMA1

OMIM

602854 (Human)

UniProt

AAH02577 (Human)

Additional PSMA1 Products

Product Documents for PSMA1 Antibody (1D9-1C7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PSMA1 Antibody (1D9-1C7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for PSMA1 Antibody (1D9-1C7) - Azide and BSA Free

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Protocols

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FAQs

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