PTH Antibody (BGN/1F8) - BSA Free

Novus Biologicals | Catalog # NB100-65676

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BGN/1F8

Format

BSA Free
View all PTH Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 1-34 of mature PTH conjugated to a proprietary carrier molecule

Reactivity Notes

Predicted cross-reactivities: Mouse, Rat

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

2 x 10^7with 1-34 PTH, 5 x 10^8 with 1-84 PTH

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for PTH Antibody (BGN/1F8) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27537691)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTH

Parathyroid hormone is secreted by parathyroid cells. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH). Most PTH is secreted in its intact, mature 9.5 kDa form, amino acids 1-84; however, it can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. No PTH fragment has been found to be the result of another gene or alternative splicing of the PTH gene.

Long Name

Parathyroid Hormone

Alternate Names

Parathormone, Parathyrin

Entrez Gene IDs

5741 (Human)

Gene Symbol

PTH

UniProt

P01270

Additional PTH Products

Product Documents for PTH Antibody (BGN/1F8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PTH Antibody (BGN/1F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PTH Antibody (BGN/1F8) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PTH Antibody (BGN/1F8) - BSA Free

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  • Q: What carrier molecule is conjugated to the immunogen of this PTH antibody (NB100-65676)? How do I know what to use for blocking? As I typically use BSA, could you recommend a blocking agent if to use this antibody for an ELISA?

    A: Unfortunately the blocking agent is proprietary, but the carrier molecule is not BSA so you can block as you normally would.

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