PTPN12 Antibody (4G6) - Azide and BSA Free

Novus Biologicals | Catalog # H00005782-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G6

Format

Azide and BSA Free
View all PTPN12 Primary Antibodies »

Product Specifications

Immunogen

PTPN12 (NP_002826.2, 682 a.a. ~ 779 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PESFVLASEHNTPVRSEWSELQSQERSEQKKSEGLITSENEKCDHPAGGIHYEMCIECPPTFSDKREQISENPTEATDIGFGNRCGKPKGPRDPPSEW

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PTPN12 Antibody (4G6) - Azide and BSA Free

Western Blot: PTPN12 Antibody (4G6) [H00005782-M01]

Western Blot: PTPN12 Antibody (4G6) [H00005782-M01]

Western Blot: PTPN12 Antibody (4G6) [H00005782-M01] - Analysis of PTPN12 expression in transfected 293T cell line by PTPN12 monoclonal antibody (M01), clone 4G6.Lane 1: PTPN12 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PTPN12 Antibody (4G6) [H00005782-M01]

Immunocytochemistry/ Immunofluorescence: PTPN12 Antibody (4G6) [H00005782-M01]

Immunocytochemistry/Immunofluorescence: PTPN12 Antibody (4G6) [H00005782-M01] - Analysis of monoclonal antibody to PTPN12 on HeLa cell. Antibody concentration 10 ug/ml.

Applications for PTPN12 Antibody (4G6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PTPN12

PTPN12 is encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. This PTP was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobility.

Long Name

Tyrosine-protein phosphatase non-receptor type 12

Alternate Names

PTP-PEST, PTPG1

Gene Symbol

PTPN12

UniProt

NP_002826 (Human)

Additional PTPN12 Products

Product Documents for PTPN12 Antibody (4G6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PTPN12 Antibody (4G6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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