PUM3 Antibody
Novus Biologicals | Catalog # H00009933-B01
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Mouse IgG
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Product Specifications
Immunogen
KIAA0020 (AAH16137.2, 1 a.a. - 648 a.a.) full-length human protein. MEVKGKKQFTGKNTKTAQEKNRFHKNSDSGSSKTFPTRKVAKEGGPKVTSRNFEKSITKLGKKGVKQFKNKQQGDKSPKNKFQPANKFNKKRKFQPDGRSDESAAKKPKWDDFKKKKKELKQSRQLSDKTNYDIVVRAKQMWEILRRKDCDKEKRVKLMSDLQKLIQGKIKTIAFAHDSTRVIQCYIQYGNEEQRKQAFEELRDDLVELSKAKYSRNIVKKFLMYGSKPQIAEIIRSFKGHVRKMLRHAEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSADHPTLDKVLELQPEKLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKLRSEMIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDTKLVKQIIISEIISSLPSIVNDKYGRKVLLYLLSPRDPAHTVREIIEVLQKGDGNAHSKKDTEVRRRELLESISPALLSYLQEHAQEVVLDKSACVLVSDILGSATGDVQPTMNAIASLAATGLHPGGKDGELHIAEHPAGHLVLKWLIEQDKKMKENGREGCFAKTLVEHVGMKNLKSWASVNRGAIILSSLLQSCDLEVANKVKAALKSLIPTLEKTKSTSKGIEILLEKLST
Specificity
KIAA0020 - KIAA0020,
Clonality
Polyclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for PUM3 Antibody
Western Blot: PUM3 Antibody [H00009933-B01]
Western Blot: KIAA0020 Antibody [H00009933-B01] - Analysis of KIAA0020 expression in transfected 293T cell line by KIAA0020 polyclonal antibody. Lane 1: KIAA0020 transfected lysate(71.28 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: PUM3 Antibody [H00009933-B01]
Immunocytochemistry/Immunofluorescence: KIAA0020 Antibody [H00009933-B01] - Analysis of purified antibody to KIAA0020 on HeLa cell. (antibody concentration 10 ug/ml)Western Blot: PUM3 Antibody [H00009933-B01]
Western Blot: KIAA0020 Antibody [H00009933-B01] - Western Blot analysis of KIAA0020 expression in transfected 293T cell line by KIAA0020 MaxPab polyclonal antibody (B01). Lane 1: KIAA0020 transfected lysate (73.60 KDa). Lane 2: Non-transfected lysate.Applications for PUM3 Antibody
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
No additive
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PUM3
Alternate Names
HBV X-transactivated gene 5 protein, HLA-HA8MGC8749, KIAA0020, KIAA0020 protein, Minor histocompatibility antigen HA-8, PEN, penguin homolog, protein 5 transactivated by hepatitis B virus X antigen (HBxAg), PUF6, XTP5HBV XAg-transactivated protein 5
Entrez Gene IDs
9933 (Human)
Gene Symbol
KIAA0020
OMIM
609960 (Human)
UniProt
Additional PUM3 Products
Product Documents for PUM3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PUM3 Antibody
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PUM3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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