Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Novus Biologicals | Catalog # NBP3-48658

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Feline, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1531
View all Puromycin-sensitive aminopeptidase/NPEPPS Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Puromycin-sensitive aminopeptidase/NPEPPS. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531) [NBP3-48658] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] (NBP3-48658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Immunohistochemistry-Paraffin: Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531) [NBP3-48658] -

Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] detects Puromycin-sensitive aminopeptidase/NPEPPS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Puromycin-sensitive aminopeptidase/NPEPPS stained by Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] (NBP3-48658) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Immunocytochemistry/ Immunofluorescence: Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531) [NBP3-48658] -

Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] detects Puromycin-sensitive aminopeptidase/NPEPPS protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Puromycin-sensitive aminopeptidase/NPEPPS stained by Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] (NBP3-48658) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531) [NBP3-48658] -

Whole zebrafish extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] (NBP3-48658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531) [NBP3-48658] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] (NBP3-48658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Immunohistochemistry-Paraffin: Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531) [NBP3-48658] -

Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] detects Puromycin-sensitive aminopeptidase/NPEPPS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Puromycin-sensitive aminopeptidase/NPEPPS stained by Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] (NBP3-48658) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531) [NBP3-48658] -

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Puromycin-sensitive aminopeptidase/NPEPPS antibody [HL1531] (NBP3-48658) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Puromycin-sensitive aminopeptidase/NPEPPS

NPEPPS encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]

Alternate Names

AAP-S, MP100, NPEPPS

Gene Symbol

NPEPPS

Additional Puromycin-sensitive aminopeptidase/NPEPPS Products

Product Documents for Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

Certificate of Analysis

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Product Specific Notices for Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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Protocols

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FAQs

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