PXR/NR1I2 Antibody (1D12G1) - BSA Free

Novus Biologicals | Catalog # NBP2-61816

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1D12G1

Format

BSA Free
View all PXR/NR1I2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PXR/NR1I2 (AA: 1-142) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PXR/NR1I2 Antibody (1D12G1) - BSA Free

Western Blot: PXR/NR1I2 Antibody (1D12G1)BSA Free [NBP2-61816]

Western Blot: PXR/NR1I2 Antibody (1D12G1)BSA Free [NBP2-61816]

Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against human NR1I2 (AA: 1-142) recombinant protein. (Expected MW is 42.2 kDa)
Immunocytochemistry/ Immunofluorescence: PXR/NR1I2 Antibody (1D12G1) - BSA Free [NBP2-61816]

Immunocytochemistry/ Immunofluorescence: PXR/NR1I2 Antibody (1D12G1) - BSA Free [NBP2-61816]

Immunocytochemistry/Immunofluorescence: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis of Hela cells using NR1I2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 Secondary antibody was used.
Western Blot: PXR/NR1I2 Antibody (1D12G1)BSA Free [NBP2-61816]

Western Blot: PXR/NR1I2 Antibody (1D12G1)BSA Free [NBP2-61816]

Western Blot: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis using NR1I2 mAb against HEK293 (1) and NR1I2 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: PXR/NR1I2 Antibody (1D12G1) - BSA Free [NBP2-61816]

ELISA: PXR/NR1I2 Antibody (1D12G1) - BSA Free [NBP2-61816]

ELISA: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
ELISA: PXR/NR1I2 Antibody (1D12G1) - BSA Free [NBP2-61816]

ELISA: PXR/NR1I2 Antibody (1D12G1) - BSA Free [NBP2-61816]

ELISA: PXR/NR1I2 Antibody (1D12G1) [NBP2-61816] - Analysis of HepG2 cells using NR1I2 mouse mAb (green) and negative control (red).

Applications for PXR/NR1I2 Antibody (1D12G1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PXR/NR1I2

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.;

Long Name

Pregnane X Receptor

Alternate Names

BXR, NR1I2, ONR1, PAR1, PAR2, SXR

Gene Symbol

NR1I2

Additional PXR/NR1I2 Products

Product Documents for PXR/NR1I2 Antibody (1D12G1) - BSA Free

Certificate of Analysis

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Product Specific Notices for PXR/NR1I2 Antibody (1D12G1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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