PYK2/FAK2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-91228
![Western Blot: PYK2/FAK2 Antibody [NBP1-91228]](https://resources.rndsystems.com/images/products/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0011.jpg "Western Blot: PYK2/FAK2 Antibody [NBP1-91228]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPM
Reactivity Notes
Mouse (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PYK2/FAK2 Antibody - BSA Free
![Western Blot: PYK2/FAK2 Antibody [NBP1-91228]](https://resources.rndsystems.com/images/products/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0013.jpg "Western Blot: PYK2/FAK2 Antibody [NBP1-91228]")
Western Blot: PYK2/FAK2 Antibody [NBP1-91228]
Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of phospho-PYK2 and total PYK2 were detected by Western blot. Image submitted by a verified customer review.![Western Blot: PYK2/FAK2 Antibody [NBP1-91228]](https://resources.rndsystems.com/images/products/PYK2-FAK2-Antibody-Western-Blot-NBP1-91228-img0012.jpg "Western Blot: PYK2/FAK2 Antibody [NBP1-91228]")
Western Blot: PYK2/FAK2 Antibody [NBP1-91228]
Western Blot: PYK2/FAK2 Antibody [NBP1-91228] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![PYK2/FAK2 Antibody - BSA Free Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]](https://resources.rndsystems.com/images/products/nbp1-91228_rabbit-polyclonal-pyk2-fak2-antibody-2952025162430.jpg "Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]")
Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]
Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![PYK2/FAK2 Antibody - BSA Free Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]](https://resources.rndsystems.com/images/products/nbp1-91228_rabbit-polyclonal-pyk2-fak2-antibody-2952025167287.jpg "Western Blot: PYK2/FAK2 Antibody - BSA Free [NBP1-91228]")
![PYK2/FAK2 Antibody - BSA Free Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228]](https://resources.rndsystems.com/images/products/nbp1-91228_-immunohistochemistry-paraffin-639173132509562870.jpg "Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228]")
Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP1-91228]
Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.Applications for PYK2/FAK2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 1 review rated 4 using NBP1-91228 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PYK2/FAK2
Long Name
Proline-rich Tyrosine Kinase 2
Alternate Names
CADTK, CAKB, FADK2, FAK2, FRNK, PTK2B, RAFTK
Gene Symbol
PTK2B
Additional PYK2/FAK2 Products
Product Documents for PYK2/FAK2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PYK2/FAK2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for PYK2/FAK2 Antibody - BSA Free
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Customer Reviews for PYK2/FAK2 Antibody - BSA Free (1)
4 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Breast cancer cellsSpecies: HumanVerified Customer | Posted 09/21/2018Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of phospho-PYK2 and total PYK2 were detected by western blot.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
