Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rab4 Primary Antibodies »

Product Specifications

Immunogen

C-terminal peptide from human Rab4

Localization

Membrane|Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab4 Antibody - BSA Free

Western Blot: Rab4 Antibody [NBP3-18233]

Western Blot: Rab4 Antibody [NBP3-18233]

Western Blot: Rab4 Antibody [NBP3-18233] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Rab4 protein using Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Rab4 Antibody [NBP3-18233]

Immunocytochemistry/ Immunofluorescence: Rab4 Antibody [NBP3-18233]

Immunocytochemistry/Immunofluorescence: Rab4 Antibody [NBP3-18233] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: String nuclear and cytoplasmic staining.
Immunohistochemistry: Rab4 Antibody [NBP3-18233]

Immunohistochemistry: Rab4 Antibody [NBP3-18233]

Immunohistochemistry: Rab4 Antibody [NBP3-18233] - Immunohistochemistry analysis using Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis (cell-cell border and cytoplasmic), hair follicles and muscle.
Western Blot: Rab4 Antibody [NBP3-18233]

Western Blot: Rab4 Antibody [NBP3-18233]

Western Blot: Rab4 Antibody [NBP3-18233] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Rab4 protein using Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (NBP3-18233) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Applications for Rab4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:150

Immunohistochemistry

1:100

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS pH 7.4, 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rab4

Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Alternate Names

Oncogene RAB4, RAB4, member RAS oncogene family, RAB4A, member RAS oncogene family, RAB4HRES-1/RAB4, ras-related protein Rab-4A

Gene Symbol

RAB4A

Additional Rab4 Products

Product Documents for Rab4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Rab4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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