Rab5a Antibody (3A4) - BSA Free

Western Blot: Rab5a Antibody (3A4)BSA Free [NBP1-04340]

Western Blot: Rab5a Antibody (3A4) [NBP1-04340] - The extracts of mouse brain (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow Cytometry: Mouse Rab5a mAb (3A4) - BSA Free [NBP1-04340] -

Flow Cytometry: Mouse Rab5a mAb (3A4) - BSA Free [NBP1-04340] - Flow cytometry of purified endosomes from mouse bone marrow derived dendritic cells bearing (blue curve) or absent of (red curve) fluorescent antigen. Endosomes stained with Alex Fluor 700 conjugated to Rab5a (NBP1-04340AF700). Image from a verified customer review.

Detection of Rab5a by Immunoprecipitation.

HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of Rab5a antibody (Catalog # NBP1-04340) pre-coupled to Dynabeads protein G. Samples were washed and processed for Western Blot with Rab5a antibody. For Western Blot, Rb x Rab5a was used at 1/1000. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).