RAB9A Antibody (1E12) - Azide and BSA Free

Novus Biologicals | Catalog # H00009367-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E12

Format

Azide and BSA Free
View all RAB9A Primary Antibodies »

Product Specifications

Immunogen

RAB9A (NP_004242, 17 a.a. ~ 115 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVGKSSLMNRYVTNKFDTQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPE

Reactivity Notes

Human. Other species not tested.

Localization

Cell Membrane (lipid anchor; cytoplasmic side).

Marker

Endosome Marker

Specificity

RAB9A - RAB9A, member RAS oncogene family

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RAB9A Antibody (1E12) - Azide and BSA Free

Western Blot: RAB9A Antibody (1E12) [H00009367-M01]

Western Blot: RAB9A Antibody (1E12) [H00009367-M01]

Western Blot: RAB9A Antibody (1E12) [H00009367-M01] - RAB9A monoclonal antibody (M01), clone 1E12 Analysis of RAB9A expression in HeLa.
Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (1E12) [H00009367-M01]

Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (1E12) [H00009367-M01]

Immunocytochemistry/Immunofluorescence: RAB9A Antibody (1E12) [H00009367-M01] - Analysis of monoclonal antibody to RAB9A on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: RAB9A Antibody (1E12) [H00009367-M01]

Western Blot: RAB9A Antibody (1E12) [H00009367-M01]

Western Blot: RAB9A Antibody (1E12) [H00009367-M01] - Analysis of RAB9A over-expressed 293 cell line, cotransfected with RAB9A Validated Chimera RNAi ( Cat # H00009367-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB9A monoclonal antibody (M01), clone 1E12 (Cat # H00009367-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: RAB9A Antibody (1E12) [H00009367-M01]

Western Blot: RAB9A Antibody (1E12) [H00009367-M01]

Western Blot: RAB9A Antibody (1E12) [H00009367-M01] - Analysis of RAB9A expression in transfected 293T cell line by RAB9A monoclonal antibody (M01), clone 1E12.Lane 1: RAB9A transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: RAB9A Antibody (1E12) [H00009367-M01] - Detection limit for recombinant GST tagged RAB9A is approximately 0.1ng/ml as a capture antibody.

Applications for RAB9A Antibody (1E12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAB9A

RAB proteins are GTPases that regulate vesicular trafficking and reside in specific intracellular compartments. RAB9 has been localized to components of the endocytic/exocytic pathway. It has been implicated in the recycling of membrane receptors, such as the mannose 6-phosphate receptor from early endosomes to the trans Golgi network. Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Alternate Names

RAB9A, member RAS oncogene family, RAB9RAB9, member RAS oncogene family, ras-related protein Rab-9A

Entrez Gene IDs

9367 (Human)

Gene Symbol

RAB9A

OMIM

300284 (Human)

UniProt

NP_004242 (Human)

Additional RAB9A Products

Product Documents for RAB9A Antibody (1E12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RAB9A Antibody (1E12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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