RAB9A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87172

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RAB9A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPESFPFVILGNKIDISERQVSTEEAQAWCRDNGDYPYFETSAKDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRKP

Marker

Endosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAB9A Antibody - BSA Free

Western Blot: RAB9A Antibody [NBP1-87172]

Western Blot: RAB9A Antibody [NBP1-87172]

Western Blot: RAB9A Antibody [NBP1-87172] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172]

Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172]

Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172] - Staining in human adrenal gland and skeletal muscle tissues using anti-RAB9A antibody. Corresponding RAB9A RNA-seq data are presented for the same tissues.
Western Blot: RAB9A Antibody [NBP1-87172]

Western Blot: RAB9A Antibody [NBP1-87172]

Western Blot: RAB9A Antibody [NBP1-87172] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).
Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172]

Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172]

Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172]

Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172]

Immunohistochemistry-Paraffin: RAB9A Antibody [NBP1-87172] - Staining of human skeletal muscle shows low expression as expected.

Applications for RAB9A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAB9A

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Alternate Names

RAB9A, member RAS oncogene family, RAB9RAB9, member RAS oncogene family, ras-related protein Rab-9A

Gene Symbol

RAB9A

Additional RAB9A Products

Product Documents for RAB9A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RAB9A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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