Rabbit IgG Antibody

Catalog #: AF008
6 Citations Datasheet / COA / SDS

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AF008

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rabbit IgG Antibody Summary

Species Reactivity

Rabbit

Specificity

Detects rabbit IgG heavy and light chains. In direct ELISAs, less than 5% cross-reactivity with mouse IgG is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Rabbit IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Product Datasheet

COA

SDS

Citations for Rabbit IgG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway
Authors: N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada
Journal of dental sciences, 2023;18(2):567-576.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway
Authors: N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada
Journal of dental sciences, 2022-09-16;18(2):567-576.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington's Disease and Influences Striatal Synaptic Density
Authors: N Zarate, TA Intihar, D Yu, J Sawyer, W Tsai, M Syed, L Carlson, R Gomez-Past
International Journal of Molecular Sciences, 2021-12-04;22(23):.
Species: Human, Mouse, Transgenic Mouse
Sample Types: Cell Lysates
Applications: ChIP
Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer
Authors: S Wu, M Luo, KKW To, J Zhang, C Su, H Zhang, S An, F Wang, D Chen, L Fu
Molecular Cancer, 2021-01-18;20(1):17.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
CXC chemokine receptor 4 signaling upon co-activation with stromal cell-derived factor-1alpha and ubiquitin.
Authors: Tripathi A, Davis J, Staren D, Volkman B, Majetschak M
Cytokine, 2013-12-24;65(2):121-5.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway.
Authors: Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE
Arthritis Rheum., 2006-03-01;54(3):765-78.
Species: Human
Sample Types: Whole Tissue
Applications: IHC