Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RABEP1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human RABEP1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for RABEP1 Antibody - BSA Free

Western Blot: RABEP1 Antibody [NBP2-97637]

Western Blot: RABEP1 Antibody [NBP2-97637]

Western Blot: RABEP1 Antibody [NBP2-97637] - Anti-RABEP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 99 kDa. Observed band size: 120 kDa.
Immunoprecipitation: RABEP1 Antibody [NBP2-97637]

Immunoprecipitation: RABEP1 Antibody [NBP2-97637]

Immunoprecipitation: RABEP1 Antibody [NBP2-97637] - RABEP1 was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 ul anti-RABEP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RABEP1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 99 kDa. Observed band size: 99 kDa
RABEP1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: RABEP1 Antibody [NBP2-97637] -

Immunocytochemistry/Immunofluorescence: RABEP1 Antibody [NBP2-97637] - Immunofluorescence staining of RABEP1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97637 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for RABEP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RABEP1

Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A

Alternate Names

neurocrescin, rab GTPase-binding effector protein 1, RAB5EPRABPT5A, rabaptin, RAB GTPase binding effector protein 1, Rabaptin-4, Rabaptin-5, Rabaptin-5alpha, RABPT5rabaptin-5alpha, Renal carcinoma antigen NY-REN-17

Gene Symbol

RABEP1

Additional RABEP1 Products

Product Documents for RABEP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RABEP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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