Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair. All eukaryotic Rad23 proteins contain an amino-terminal UbL domain that bears a striking resemblance to ubiquitin. Rad23 has a proteolytic function in DNA repair via its interaction with the 26S proteosome through the UbL domain. The elimination of this Rad23 domain causes sensitivity to ultraviolet radiation.
Rad23 Antibody (3E3F4) - BSA Free
Novus Biologicals | Catalog # NBP2-52549
![Western Blot: Rad23 Antibody (3E3F4)BSA Free [NBP2-52549]](https://resources.rndsystems.com/images/products/hHR23A-Antibody-3E3F4-Western-Blot-NBP2-52549-img0004.jpg "Western Blot: Rad23 Antibody (3E3F4)BSA Free [NBP2-52549]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 3E3F4
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human Rad23 (AA: 1-363) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rad23 Antibody (3E3F4) - BSA Free
Western Blot: Rad23 Antibody (3E3F4)BSA Free [NBP2-52549]
Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against human RAD23A (AA: 1-363) recombinant protein. (Expected MW is 42.5 kDa)![Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]](https://resources.rndsystems.com/images/products/hHR23A-Antibody-3E3F4-Immunohistochemistry-Paraffin-NBP2-52549-img0003.jpg "Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]")
Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]
Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of paraffin-embedded esophageal cancer tissues using RAD23A mouse mAb with DAB staining.![Flow Cytometry: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]](https://resources.rndsystems.com/images/products/hHR23A-Antibody-3E3F4-Flow-Cytometry-NBP2-52549-img0001.jpg "Flow Cytometry: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]")
Flow Cytometry: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]
Flow Cytometry: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of HeLa cells using RAD23A mouse mAb (green) and negative control (red).![Western Blot: Rad23 Antibody (3E3F4)BSA Free [NBP2-52549]](https://resources.rndsystems.com/images/products/hHR23A-Antibody-3E3F4-Western-Blot-NBP2-52549-img0005.jpg "Western Blot: Rad23 Antibody (3E3F4)BSA Free [NBP2-52549]")
Western Blot: Rad23 Antibody (3E3F4)BSA Free [NBP2-52549]
Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against HEK293 (1) and RAD23A (AA: 1-363)-hIgGFc transfected HEK293 (2) cell lysate.![Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]](https://resources.rndsystems.com/images/products/hHR23A-Antibody-3E3F4-Immunohistochemistry-Paraffin-NBP2-52549-img0002.jpg "Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]")
Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]
Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of paraffin-embedded bladder cancer tissues using RAD23A mouse mAb with DAB staining.![ELISA: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]](https://resources.rndsystems.com/images/products/Rad23-Antibody-3E3F4-ELISA-NBP2-52549-img0006.jpg "ELISA: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]")
ELISA: Rad23 Antibody (3E3F4) - BSA Free [NBP2-52549]
ELISA: Rad23 Antibody (3E3F4) [NBP2-52549] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).Applications for Rad23 Antibody (3E3F4) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready.
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rad23
Alternate Names
hHR23
Gene Symbol
RAD23A
Additional Rad23 Products
Product Documents for Rad23 Antibody (3E3F4) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rad23 Antibody (3E3F4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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