Rad50 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-154

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Hamster

Cited:

Human, Mouse, Hamster

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, ICC/IF (Negative)

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rad50 Primary Antibodies »

Product Specifications

Immunogen

C-terminal mouse RAD50 (604 amino acids). [UniProt# P70388]

Localization

Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

153 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad50 Antibody - BSA Free

Simple Western: Rad50 Antibody [NB100-154]

Simple Western: Rad50 Antibody [NB100-154]

Simple Western: Rad50 Antibody [NB100-154] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: Rad50 Antibody [NB100-154]

Western Blot: Rad50 Antibody [NB100-154]

Rad50-Antibody-Western-Blot-NB100-154-img0006.jpg
Western Blot: Rad50 Antibody [NB100-154]

Western Blot: Rad50 Antibody [NB100-154]

Western Blot: Rad50 Antibody [NB100-154] - WB detection of RAD50 protein in 30ug whole cell lysate of 293T cells using Rad50 antibody at 1:2000 dilution (Submitted via Verified Customer Review).
Western Blot: Rad50 Antibody [NB100-154]

Western Blot: Rad50 Antibody [NB100-154]

Western Blot: Rad50 Antibody [NB100-154] - Detection of Rad50 in HeLa nuclear extracts.

Applications for Rad50 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Immunoprecipitation

1:10-1:500

Simple Western

1:2000

Western Blot

1:2000
Application Notes

This RAD50 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at 153 kDa. IP has been done with 3-4 X 10(6) cells. ICC/IF does not appear to work. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:2000, apparent MW was 151 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 4 using NB100-154 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Rad50

RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).

Alternate Names

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Entrez Gene IDs

10111 (Human); 19360 (Mouse)

Gene Symbol

RAD50

Additional Rad50 Products

Product Documents for Rad50 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Rad50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad50 Antibody - BSA Free

Customer Reviews for Rad50 Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
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  • Rad50 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: human lung cancer cell line
    Species: Human
    Verified Customer | Posted 02/18/2019
    Rad50 Antibody - BSA Free NB100-154
  • Name: Anatoli Grechov
    Application: Western Blot
    Sample Tested: 293T lysate
    Species: Human
    Verified Customer | Posted 12/17/2014
    RAD50 WB in 293T cells
    Rad50 Antibody - BSA Free NB100-154

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