Rad50 Antibody

Novus Biologicals | Catalog # NBP2-20054

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (94%), Rabbit (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Rad50 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rad50. The exact sequence is proprietary.

Localization

Nucleus; Telomere

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

154 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad50 Antibody

Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20054]

Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20054]

Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20054] - Human colon carcinoma, using Rad50 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Rad50 Antibody [NBP2-20054]

Western Blot: Rad50 Antibody [NBP2-20054]

Western Blot: RAD50 Antibody [NBP2-20054] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: Rad50 Antibody [NBP2-20054]

Western Blot: Rad50 Antibody [NBP2-20054]

Western Blot: Rad50 Antibody [NBP2-20054] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal.
Rad50 Antibody

Western Blot: Rad50 Antibody [NBP2-20054] -

Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (NBP2-20054) diluted at 1:1000.
Rad50 Antibody

Western Blot: Rad50 Antibody [NBP2-20054] -

Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal (NBP2-20054) diluted at 1:5000.
Rad50 Antibody

Western Blot: Rad50 Antibody [NBP2-20054] -

Mre11 hypomorphism alters DSB repair pathway utilization. (A) Schema depicting the role of Mre11-mediated end resection in the regulation of DSB repair pathway choice. (B) Mre11ATLD1/ATLD1 MEFs exhibit reduced expression of the MRN complex by immunoblotting. (C–F) DSB repair products were identified in WT, Mre11ATLD1-1 and Mre11ATLD1-2 cells. (C) TMEJ-del 23 bp. (D) TMEJ-del 39 bp. (E) HR repair (HRD-500 donor) and (F) NHEJ-ins +1 bp. Mean +/- SEM are shown. Statistical significance was assessed by two-tailed t-tests. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33963863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Rad50 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000
Application Notes
Use in KD reported in scientific publication (PMID: 32023457).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad50

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]

Alternate Names

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Gene Symbol

RAD50

UniProt

Q92878

Additional Rad50 Products

Product Documents for Rad50 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Rad50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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