Rad50 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20055

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rad50 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Rad50. The exact sequence is proprietary.

Localization

Nucleus; Telomere

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

154 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad50 Antibody - BSA Free

Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20055]

Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-20055]

Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20055] - Immunohistochemical analysis of paraffin-embedded Lung carcinoma, using antibody at 1:250 dilution.
Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: RAD50 Antibody [NBP2-20055] - Rad50 antibody [N1N2], N-term detects RAD50 protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract B. 30 ug GL261 whole cell lysate/extract5 % SDS-PAGERad50 antibody [N1N2], N-term dilution: 1:1000.
Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: RAD50 Antibody [NBP2-20055] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: Rad50 Antibody [NBP2-20055] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: Rad50 Antibody [NBP2-20055] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: Rad50 Antibody [NBP2-20055]

Western Blot: Rad50 Antibody [NBP2-20055] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Rad50 Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

1:100 - 1:2000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad50

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]

Alternate Names

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Gene Symbol

RAD50

UniProt

Q92878

Additional Rad50 Products

Product Documents for Rad50 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rad50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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