Rad51 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00005888-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bacteria

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all Rad51 Primary Antibodies »

Product Specifications

Immunogen

RAD51 (NP_002866.2, 1 a.a. - 339 a.a.) full-length human protein. MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

RAD51 - RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae),

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Rad51 Antibody - Azide and BSA Free

Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in human colon.
Immunocytochemistry/ Immunofluorescence: Rad51 Antibody [H00005888-B01P]

Immunocytochemistry/ Immunofluorescence: Rad51 Antibody [H00005888-B01P]

Immunocytochemistry/Immunofluorescence: Rad51 Antibody [H00005888-B01P] - Immunofluorescence of purified MaxPab antibody to RAD51 on HeLa cell. [antibody concentration 10 ug/ml]
Immunohistochemistry-Paraffin: Rad51 Antibody [H00005888-B01P]

Immunohistochemistry-Paraffin: Rad51 Antibody [H00005888-B01P]

Immunohistochemistry-Paraffin: Rad51 Antibody [H00005888-B01P] - Analysis of purified antibody to RAD51 on formalin-fixed paraffin-embedded human testis. (antibody concentration 3 ug/ml)
Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P] - expression in transfected 293T cell line. Lane 1: RAD51 transfected lysate(37.29 KDa). Lane 2: Non-transfected lysate.
Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in PC-12.
Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in Raw 264.7.
Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in Jurkat.
Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in NIH/3T3.
Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P]

Western Blot: Rad51 Antibody [H00005888-B01P] - Analysis of RAD51 expression in transfected 293T cell line by RAD51 polyclonal antibody. Lane 1: RAD51 transfected lysate(37.29 KDa). Lane 2: Non-transfected lysate.

Applications for Rad51 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. May also be used for IF, IHC-P. Use in Immunohistochemistry reported in scientific literature (PMID: 26675349).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad51

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq]

Alternate Names

BRCC5, hRAD51, HsRad51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A

Entrez Gene IDs

5888 (Human)

Gene Symbol

RAD51

UniProt

NP_002866.2 (Human)

Additional Rad51 Products

Product Documents for Rad51 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rad51 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad51 Antibody - Azide and BSA Free

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Protocols

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