Rad51L1 Antibody (1 H3/13) - BSA Free

Novus Biologicals | Catalog # NB100-176

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative), Primate

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1 H3/13

Format

BSA Free
View all Rad51L1 Primary Antibodies »

Product Specifications

Immunogen

His-tagged human Rad51L1, overexpressed in E. coli. [UniProt# O15315]

Reactivity Notes

This antibody does not work in mouse.

Specificity

Does not cross-react with Rad51C, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad51L1 Antibody (1 H3/13) - BSA Free

Western Blot: Rad51L1 Antibody (1 H3/13)BSA Free [NB100-176]

Western Blot: Rad51L1 Antibody (1 H3/13)BSA Free [NB100-176]

Western Blot: Rad51L1 Antibody (1 H3/13) [NB100-176] - Western blot analysis of Rad51L1 expression in 1) HeLa, 2) HepG2 and 3) Cos 7 whole cell lysates.

Applications for Rad51L1 Antibody (1 H3/13) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Western Blot

1:1000
Application Notes
In Western blot, a band can be seen at ~ 42 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad51L1

Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. Rad51B plays a role in meiotic recombination and/or recombinational repair.

Alternate Names

DNA repair protein RAD51 homolog 2, MGC34245, R51H2REC2hREC2, RAD51 (S. cerevisiae)-like 1, RAD51 homolog B, RAD51B, RAD51-like 1 (S. cerevisiae), RAD51-like protein 1, RecA-like protein, recombination repair protein

Entrez Gene IDs

5890 (Human); 19363 (Mouse)

Gene Symbol

RAD51B

UniProt

O15315 (Human)

Additional Rad51L1 Products

Product Documents for Rad51L1 Antibody (1 H3/13) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rad51L1 Antibody (1 H3/13) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad51L1 Antibody (1 H3/13) - BSA Free

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Protocols

View specific protocols for Rad51L1 Antibody (1 H3/13) - BSA Free (NB100-176):

Procedure Guide for NB 100-176 Anti-Rad51B Rad51L1 Antibody (1 H3/13):


Western Blot
1. Proteins are separated on an SDS-PAGE gel.

2. Following the protein transfer, the membrane is blocked with 5% NFDM in TBS-T.

3. The membrane is then washed.

4. NB 100-176 [anti-Rad51B] is diluted 1:1,000 in dilution buffer [5% NFDM in TBS-T] and incubated for 3 hours at room temperature, gently shaking.

5. The membrane is then washed.

6. Secondary antibody [HRP conjugated goat anti-mouse] is incubated for 45 minutes at room temperature, gently shaking.

7. Develop membrane.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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