RalB Antibody (4D1) - Azide and BSA Free

Novus Biologicals | Catalog # H00005899-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4D1

Format

Azide and BSA Free
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Product Specifications

Immunogen

RALB (AAH18163, 89 a.a. ~ 183 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEERRQVPVEEARSKAEEWGVQYVETSAKTRANVDKVFFDLMREIRTKKMSE

Specificity

RALB (4D1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RalB Antibody (4D1) - Azide and BSA Free

Western Blot: RalB Antibody (4D1) [H00005899-M04]

Western Blot: RalB Antibody (4D1) [H00005899-M04]

Western Blot: RalB Antibody (4D1) [H00005899-M04] - Analysis of RALB expression in transfected 293T cell line by RALB monoclonal antibody (M04), clone 4D1. Lane 1: RALB transfected lysatE (23.4 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: RalB Antibody (4D1) [H00005899-M04]

Immunohistochemistry-Paraffin: RalB Antibody (4D1) [H00005899-M04]

Immunohistochemistry-Paraffin: RalB Antibody (4D1) [H00005899-M04] - Analysis of monoclonal antibody to RALB on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
Immunoprecipitation: RalB Antibody (4D1) [H00005899-M04]

Immunoprecipitation: RalB Antibody (4D1) [H00005899-M04]

Immunoprecipitation: RalB Antibody (4D1) [H00005899-M04] - Analysis of RALB transfected lysate using anti-RALB monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RALB rabbit polyclonal antibody.
ELISA: RalB Antibody (4D1) [H00005899-M04]

ELISA: RalB Antibody (4D1) [H00005899-M04]

ELISA: RalB Antibody (4D1) [H00005899-M04] - Detection limit for recombinant GST tagged RALB is approximately 0.3ng/ml as a capture antibody.

Applications for RalB Antibody (4D1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RalB

This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.

Long Name

Ras-like Protein B

Alternate Names

GTP binding protein, RAS-like protein B, ras-related protein Ral-B, v-ral simian leukemia viral oncogene homolog B (ras related; GTP bindingprotein)

Entrez Gene IDs

5899 (Human)

Gene Symbol

RALB

OMIM

179551 (Human)

Additional RalB Products

Product Documents for RalB Antibody (4D1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RalB Antibody (4D1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RalB Antibody (4D1) - Azide and BSA Free

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Protocols

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FAQs

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