RalB Antibody (7F8B4) - BSA Free
Novus Biologicals | Catalog # NBP2-61831
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 7F8B4
Format
BSA Free
Loading...
Product Specifications
Immunogen
Purified recombinant fragment of human RALB (AA: 89-206) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse RalB Antibody (7F8B4) - BSA Free (NBP2-61831) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RalB Antibody (7F8B4) - BSA Free
Western Blot: RalB Antibody (7F8B4)BSA Free [NBP2-61831]
Western Blot: RalB Antibody (7F8B4) [NBP2-61831] - Analysis using RALB mAb against HEK293 (1) and RALB (AA: 89-206)-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry: RalB Antibody (7F8B4) - BSA Free [NBP2-61831]
Immunohistochemistry: RalB Antibody (7F8B4) [NBP2-61831] - Analysis of paraffin-embedded bladder cancer tissues using RALB mouse mAb with DAB staining.Western Blot: RalB Antibody (7F8B4)BSA Free [NBP2-61831]
Western Blot: RalB Antibody (7F8B4) [NBP2-61831] - Analysis using RALB mAb against human RALB (AA: 89-206) recombinant protein. (Expected MW is 39.7 kDa)Western Blot: RalB Antibody (7F8B4)BSA Free [NBP2-61831]
Western Blot: RalB Antibody (7F8B4) [NBP2-61831] - Analysis using RALB mouse mAb against HepG2 (1), COS7 (2), NIH/3T3 (3), A549 (4), U251 (5), HT-29 (6), HEK293 (7), and LOVO (8) cell lysate.Immunohistochemistry: RalB Antibody (7F8B4) - BSA Free [NBP2-61831]
Immunohistochemistry: RalB Antibody (7F8B4) [NBP2-61831] - Analysis of paraffin-embedded cervical cancer tissues using RALB mouse mAb with DAB staining.ELISA: RalB Antibody (7F8B4) - BSA Free [NBP2-61831]
ELISA: RalB Antibody (7F8B4) [NBP2-61831] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)ELISA: RalB Antibody (7F8B4) - BSA Free [NBP2-61831]
ELISA: RalB Antibody (7F8B4) [NBP2-61831] - Analysis of Hela cells using RALB mouse mAb (green) and negative control (red).Applications for RalB Antibody (7F8B4) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:100-1:2000
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RalB
Long Name
Ras-like Protein B
Alternate Names
GTP binding protein, RAS-like protein B, ras-related protein Ral-B, v-ral simian leukemia viral oncogene homolog B (ras related; GTP bindingprotein)
Gene Symbol
RALB
Additional RalB Products
Product Documents for RalB Antibody (7F8B4) - BSA Free
Product Specific Notices for RalB Antibody (7F8B4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for RalB Antibody (7F8B4) - BSA Free
There are currently no reviews for this product. Be the first to review RalB Antibody (7F8B4) - BSA Free and earn rewards!
Have you used RalB Antibody (7F8B4) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...