Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C4

Format

Azide and BSA Free
View all RalB Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RALB(NP_002872) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse RalB Antibody (OTI2C4) - Azide and BSA Free (NBP2-45927) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RalB Antibody (OTI2C4) - Azide and BSA Free

Western Blot: RalB Antibody (OTI2C4)Azide and BSA Free [NBP2-73785]

Western Blot: RalB Antibody (OTI2C4)Azide and BSA Free [NBP2-73785]

Western Blot: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALB.
Immunocytochemistry/ Immunofluorescence: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785]

Immunocytochemistry/ Immunofluorescence: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785]

Immunocytochemistry/Immunofluorescence: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RALB.
Immunohistochemistry: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785]

Immunohistochemistry: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785]

Immunohistochemistry: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: RalB Antibody (OTI2C4)Azide and BSA Free [NBP2-73785]

Western Blot: RalB Antibody (OTI2C4)Azide and BSA Free [NBP2-73785]

Western Blot: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785]

Immunohistochemistry: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785]

Immunohistochemistry: RalB Antibody (OTI2C4) - Azide and BSA Free [NBP2-73785] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for RalB Antibody (OTI2C4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RalB

The Ras-like proteins RalA and RalB are homologous proteins that constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral protein activity is regulated by cycling between an active GTP-bound and an inactive GDP-bound state. Ral GTPases have been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name

Ras-like Protein B

Alternate Names

GTP binding protein, RAS-like protein B, ras-related protein Ral-B, v-ral simian leukemia viral oncogene homolog B (ras related; GTP bindingprotein)

Gene Symbol

RALB

Additional RalB Products

Product Documents for RalB Antibody (OTI2C4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RalB Antibody (OTI2C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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