Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RanGAP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human Ran GTPase activating protein 1 using the numbering given in entry NP_002874.1 (GeneID 5905).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit RanGAP1 Antibody - BSA Free (NBP1-18915) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RanGAP1 Antibody - BSA Free

Western Blot: RanGAP1 Antibody [NBP1-18915]

Western Blot: RanGAP1 Antibody [NBP1-18915]

Western Blot: RanGAP1 Antibody [NBP1-18915] - Detection of human and mouse RanGAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: RanGAP1 Antibody [NBP1-18915]

Immunohistochemistry: RanGAP1 Antibody [NBP1-18915]

Immunohistochemistry: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RanGAP1 Cat. No. NBP1-18915 (left) and another rabbit anti-RanGAP1 antibody (right) used at a dilution 1ug/ml. Detection: DAB
Western Blot: RanGAP1 Antibody [NBP1-18915]

Western Blot: RanGAP1 Antibody [NBP1-18915]

Western Blot: RanGAP1 Antibody [NBP1-18915] - Whole cell lysate from 293T and HeLa cells. RanGAP1 was also immunoprecipitated by rabbit anti-RanGAP1 antibody NBP1-18916.
Immunoprecipitation: RanGAP1 Antibody [NBP1-18915]

Immunoprecipitation: RanGAP1 Antibody [NBP1-18915]

Immunoprecipitation: RanGAP1 Antibody [NBP1-18915] - Detection of human RanGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanGAP1 antibody NBP1-18915 used for IP at 6 ug per reaction. RanGAP1 was also immunoprecipitated by another rabbit anti-RanGAP1 antibody. For blotting immunoprecipitated RanGAP1, NBP1-18915 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RanGAP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RanGAP1

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

Long Name

Ran GTPase Activating Protein 1

Alternate Names

Fug1

Entrez Gene IDs

5905 (Human)

Gene Symbol

RANGAP1

UniProt

P46060 (Human)

Additional RanGAP1 Products

Product Documents for RanGAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RanGAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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