RAP1A Antibody (1D4-1A3)

Novus Biologicals | Catalog # NBP2-22527

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Cited:

Western Blot, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1D4-1A3
View all Rap1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Rap1

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30002060).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for RAP1A Antibody (1D4-1A3)

Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.
Immunocytochemistry/ Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Immunocytochemistry/ Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).
Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527]

Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

Applications for RAP1A Antibody (1D4-1A3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:500

Immunoprecipitation

2 ug

Western Blot

1:500 - 1:1000
Application Notes
Use in Proximity Ligation Assay reported in scientific literature (PMID:30002060).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA and 30% glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rap1A

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Ras-related Protein 1A

Alternate Names

KREV-1, SMGP21

Entrez Gene IDs

5906 (Human)

Gene Symbol

RAP1A

UniProt

P62834

Additional Rap1A Products

Product Documents for RAP1A Antibody (1D4-1A3)

Certificate of Analysis

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Product Specific Notices for RAP1A Antibody (1D4-1A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAP1A Antibody (1D4-1A3)

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Protocols

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