RAR alpha/NR1B1 Antibody (763) - Azide Free

Novus Biologicals | Catalog # NB200-322

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Guinea Pig (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 763

Format

Azide Free
View all RAR alpha/NR1B1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P10276

Reactivity Notes

Tested in Human. Mouse reactivity reported in scientific literature (PMID: 24504337) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. It is anticipated that the antibody will also work with bovine, canine, and guinea pig tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~48 kDa RAR-alpha isotype.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAR alpha/NR1B1 Antibody (763) - Azide Free

Western Blot: RAR alpha/NR1B1 Antibody (763) [NB200-322]

Western Blot: RAR alpha/NR1B1 Antibody (763) [NB200-322]

Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.

Applications for RAR alpha/NR1B1 Antibody (763) - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Western blot where a ~48 kDa band can be seen using rat brain hippocampal homogenate. (Tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3.) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24504337). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:24504337).

Reviewed Applications

Read 1 review rated 3 using NB200-322 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAR alpha/NR1B1

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Retinoic acid receptor alpha mRNA expression levels are significantly higher in Barrett's tissues compared with normal esophagus tissues (3). In addition, RAR alpha controls the action of all-trans retinoic acid in gastric cancer cells (4).

Long Name

Retinoic Acid Receptor, alpha

Alternate Names

NR1B1, RARA

Entrez Gene IDs

5914 (Human); 24705 (Rat)

Gene Symbol

RARA

UniProt

P10276 (Human)

Additional RAR alpha/NR1B1 Products

Product Documents for RAR alpha/NR1B1 Antibody (763) - Azide Free

Certificate of Analysis

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Product Specific Notices for RAR alpha/NR1B1 Antibody (763) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAR alpha/NR1B1 Antibody (763) - Azide Free

Customer Reviews for RAR alpha/NR1B1 Antibody (763) - Azide Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell lysate from MOLT-4 cells, in lysis buffer
    Species: Human
    Verified Customer | Posted 09/11/2014
    WB for RARalpha in MOLT-4 cells
    RAR alpha/NR1B1 Antibody (763) - Azide Free NB200-322

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