RASA3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20081

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (92%), Chicken (90%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RASA3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RASA3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RASA3 Antibody - BSA Free

Immunohistochemistry-Paraffin: RASA3 Antibody [NBP2-20081]

Immunohistochemistry-Paraffin: RASA3 Antibody [NBP2-20081]

Immunohistochemistry-Paraffin: RASA3 Antibody [NBP2-20081] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.
RASA3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RASA3 Antibody [NBP2-20081] -

RASA3 antibody [C2C3], C-term detects RASA3 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RASA3 protein stained by RASA3 antibody [C2C3], C-term (NBP2-20081) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
RASA3 Antibody - BSA Free

Western Blot: RASA3 Antibody [NBP2-20081] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RASA3 antibody [C2C3], C-term (NBP2-20081) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RASA3 Antibody - BSA Free

Western Blot: RASA3 Antibody [NBP2-20081] -

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RASA3 antibody [C2C3], C-term (NBP2-20081) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RASA3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RASA3

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This family member is an inositol 1,3,4,5-tetrakisphosphate-binding protein, like the closely related RAS p21 protein activator 2. The two family members have distinct pleckstrin-homology domains, with this particular member having a domain consistent with its localization to the plasma membrane. [provided by RefSeq]

Alternate Names

GAP1(IP4BP), GAP1IP4BP, GAPIII, GTPase activating protein III, inositol 13,4,5-tetrakisphosphate-binding protein, Ins P4-binding protein, Ins(13,4,5)P4-binding protein, MGC46517, MGC47588, ras GTPase-activating protein 3, RAS p21 protein activator 3

Gene Symbol

RASA3

UniProt

Q14644 (Human)

Additional RASA3 Products

Product Documents for RASA3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RASA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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