RASAL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32776

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Knockdown Validated

Cited:

Western Blot, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RASAL1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RASAL1. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31641113). Monkey reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RASAL1 Antibody - BSA Free

Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776]

RASAL1-Antibody-Western-Blot-NBP1-32776-img0006.jpg
Immunocytochemistry/ Immunofluorescence: RASAL1 Antibody [NBP1-32776]

Immunocytochemistry/ Immunofluorescence: RASAL1 Antibody [NBP1-32776]

Immunocytochemistry/Immunofluorescence: RASAL1 Antibody [NBP1-32776] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:1000.
Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776] - Protein expression was upregulated in older female crab-eating monkey brain. Image submitted by a verified customer review.
RASAL1 Antibody

Western Blot: RASAL1 Antibody [NBP1-32776] -

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits in vitro anti-CD3 & dendritic cell-peptide induced T-cell activation. a Antigen (OVA323-39)-dependent activation & proliferation of DO11.10 CD4 + cells expressing Flag-tagged Rasal1 compared with control cells at different OVA peptide concentrations (5–10 ng). CTLA-Ig was used as a control. Upper inset shows expression of Flag-tagged Rasal using anti-Flag in blotting (n = 3). b Inhibition of T-cell responses by Rasal1 to increasing anti-CD3 concentrations. Upper inset: effects of Rasal1 at 48 & 72 h post-anti-CD3 activation (n = 3). c Anti-CD3-driven NFATc1/AP1 promoter activity in Jurkat T cells was inhibited by the presence of Flag-tagged Rasal1 (n = 3). d siRNA (esiRNA) knockdown of Rasal1 enhances T-cell proliferation. esiRNA were tested for their knockdown efficiency by western blot (top right panel), esiRNA1 & 2 correspond to 500 nM & 1000 nM concentrations (n = 3). e Contact time between D011.10 CD4+T-cells expressing Flag-Rasal1 (red) or empty vector (blue) & OVA peptide on bone marrow dendritic cells (BMDCs). Dot blot of T cell/DC contact duration time in presence of OVA was unaffected by Flag-tagged Rasal1 or vector transfection (n = 3). Representative plots show mean with ± SD. p-value was calculated by unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RASAL1 Antibody

Western Blot: RASAL1 Antibody [NBP1-32776] -

Western Blot: RASAL1 Antibody [NBP1-32776] - TCR complex purification & Rasal1 association with ZAP-70 in the TCR complex. a Analytic gel from tandem affinity purification (TAP) of TCR plus associated proteins captured from TCR-constituted cells (TCR complex) versus TCR-null negative control cells (control) (left panel). Chart showing the list of TCR-associated proteins identified by MS analysis (right upper panel). The structure of Rasal1 (right lower panel). b Co-precipitation of ZAP-70 with TCR from 3A9 T cells. 3A9 cells were stimulated for 10 min with anti-CD3 followed by immunoprecipitation with anti-TCR zeta & blotting with anti-ZAP-70 or anti-zeta. Lane 1: resting T cells; lane 2: anti-CD3-stimulated T cells. c Rasal1 expression is induced with anti-CD3 activation. Relative fold change expression of Rasal1 versus Rasal3 upon anti-CD3 stimulation of CD4+ & CD8 + peripheral T cells. 18 s rRNA served as a control. d Anti-CD3 induces Rasal1 translocation to the membranes of T cells. Stimulation-dependent association of Rasal1 with the membrane (upper panel) versus cytosolic Rasal1 (lower panel). Anti-actin served as a loading control. LAT was used as a marker of membrane enrichment fractionation (n = 3) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RASAL1 Antibody

Western Blot: RASAL1 Antibody [NBP1-32776] -

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RASAL1 Antibody

Western Blot: RASAL1 Antibody [NBP1-32776] -

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RASAL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
RASAL1 antibody validated for WB from a verified customer review. Use in Proximity Ligation Assay reported in scientific literature (PMID:31641113).

Reviewed Applications

Read 2 reviews rated 3.5 using NBP1-32776 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RASAL1

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues.

Alternate Names

RAS protein activator like 1 (GAP1 like), RASALGAP1 like protein, rasGAP-activating-like protein 1

Entrez Gene IDs

8437 (Human)

Gene Symbol

RASAL1

UniProt

O95294 (Human)

Additional RASAL1 Products

Product Documents for RASAL1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RASAL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for RASAL1 Antibody - BSA Free

Customer Reviews for RASAL1 Antibody - BSA Free (2)

3.5 out of 5
2 Customer Ratings
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  • RASAL1 Antibody
    Name: Hui Zhang
    Application: Western Blot
    Sample Tested: brain and spinal cord
    Species: Monkey
    Verified Customer | Posted 02/13/2019
    Protein expression was upregulated in older female crab-eating monkey brain.
    Antibody dilution 1:1000
    RASAL1 Antibody - BSA Free NBP1-32776
  • Name: Youg Thaker
    Application: Western Blot
    Sample Tested: 293T lysate transfected with hRasal1
    Species: Human
    Verified Customer | Posted 02/10/2014
    Western Blot
    RASAL1 Antibody - BSA Free NBP1-32776

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Protocols

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