RASSF1 Antibody (OTI2B11)

Novus Biologicals | Catalog # NBP2-03644

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B11
View all RASSF1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RASSF1 (NP_009113) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RASSF1 Antibody (OTI2B11)

Western Blot: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Western Blot: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.
Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (2B11) [NBP2-03644] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.
Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human prostate tissue using anti-RASSF1 mouse monoclonal antibody.
Flow Cytometry: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Flow Cytometry: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Flow Cytometry: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RASSF1 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human endometrium tissue using anti-RASSF1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (OTI2B11) [NBP2-03644]

Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human lymphoma tissue using anti-RASSF1 mouse monoclonal antibody.

Applications for RASSF1 Antibody (OTI2B11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RASSF1

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Alternate Names

123F2, NORE2A, pancreas-specific ras association domain family 1 protein, Ras association (RalGDS/AF-6) domain family member 1, ras association domain-containing protein 1, RASSF1A, RDA32cardiac-specific ras association domain family 1 protein, REH3P21, tumor suppressor protein RDA32, WUGSC:H_LUCA12.5

Entrez Gene IDs

28844 (Human)

Gene Symbol

RASSF1

Additional RASSF1 Products

Product Documents for RASSF1 Antibody (OTI2B11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RASSF1 Antibody (OTI2B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RASSF1 Antibody (OTI2B11)

Customer Reviews for RASSF1 Antibody (OTI2B11)

There are currently no reviews for this product. Be the first to review RASSF1 Antibody (OTI2B11) and earn rewards!

Have you used RASSF1 Antibody (OTI2B11)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies