The specificity of this antibody was confirmed by direct ELISA on mouse IgG2B monoclonal antibodies, and it does not cross-react with IgG1, IgG2A, IgG3, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG2B isotype, and does not detect primary antibodies of mouse IgG1, IgG2A, or IgG3 class.
Monoclonal Rat IgG1 Clone # 332723
Protein A or G purified from hybridoma culture supernatant
Cocktail of mouse IgG2B and IgG3
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Mouse IgG2B Primary Antibody by Flow Cytometry.
Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), or B) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB0041, open histogram), followed by PE-conjugated Rat Anti-Mouse IgG2B isotype-specific secondary antibody (Catalog # F0132). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Immunoglobulin G2B Fc
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