Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 866614
View all CXCR2/IL-8RB Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR2/IL-8 RB
Accession # P35407

Specificity

Detects rat CXCR2/IL-8 RB in ELISA. Stains rat CXCR2/IL-8 RB transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Rat CXCR2/IL-8RB APC-conjugated Antibody

Detection of CXCR2/IL-8 RB antibody in Rat Blood Granulocytes antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in Rat Blood Granulocytes by Flow Cytometry.

Rat peripheral blood granulocytes were stained with Mouse Anti-Rat CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB8110A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR2/IL-8 RB antibody in HEK Human Cell Line Transfected with Rat CSCR2/IL-8 RB and eGFP antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in HEK Human Cell Line Transfected with Rat CSCR2/IL-8 RB and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB8110A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat CXCR2/IL-8RB APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Rat peripheral blood granulocytes and HEK293 human embryonic kidney cell line transfected with rat CXCR2/IL-8 RB and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR2/IL-8RB

CXCR2 is an approximately 40 kDa 7-transmembrane domain receptor for the ELR+ chemokines CXCL1, 2, 3, 5, 6, 7, 8 and MIF. CXCR2 is expressed on neutrophils, monocytes, eosinophils, basophils, mast cells, T cells, oligodendrocytes, airway smooth muscle cells, and vascular endothelial cells. It is additionally upregulated in several cancers. CXCR2 can associate into homodimers or heterodimers with CXCR1, CXCR4, CD74, or the delta Opioid Receptor. CXCR2 plays an important role in attracting immune cells to sites of inflammation followed by their adhesion and extravasation. It is also involved in angiogenesis, the development of inflammatory disorders, and cancer. Rat CXCR2 shares 71% and 86% amino acid sequence identity with human and mouse CXCR2, respectively.

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Gene Symbol

CXCR2

UniProt

P35407

Additional CXCR2/IL-8RB Products

Product Documents for Rat CXCR2/IL-8RB APC-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat CXCR2/IL-8RB APC-conjugated Antibody

For research use only

Citations for Rat CXCR2/IL-8RB APC-conjugated Antibody

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