Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 866614
View all CXCR2/IL-8RB Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR2/IL-8 RB
Accession # P35407

Specificity

Detects rat CXCR2/IL-8 RB in direct ELISAs. In Flow Cytometry, stains HEK293 cells transfected with rat CXCR2/IL-8 RB, but does not stain non‑transfected cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Rat CXCR2/IL-8RB Antibody

Detection of CXCR2/IL-8 RB antibody in Rat Blood Granulocytes antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in Rat Blood Granulocytes by Flow Cytometry.

Rat peripheral blood granulocytes were stained with Mouse Anti-Rat CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB8110, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Detection of CXCR2/IL-8 RB antibody in HEK293 Human Cell Line Transfected with Rat CXCR2/IL-8 RB and eGFP antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Rat CXCR2/IL-8 RB and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB8110) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB004). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat CXCR2/IL-8RB Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Rat peripheral blood granulocytes and HEK293 human embryonic kidney cell line transfected with rat CXCR2/IL-8 RB and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR2/IL-8RB

CXCR2 is an approximately 40 kDa 7-transmembrane domain receptor for the ELR+ chemokines CXCL1, 2, 3, 5, 6, 7, 8 and MIF. CXCR2 is expressed on neutrophils, monocytes, eosinophils, basophils, mast cells, T cells, oligodendrocytes, airway smooth muscle cells, and vascular endothelial cells. It is additionally upregulated in several cancers. CXCR2 can associate into homodimers or heterodimers with CXCR1, CXCR4, CD74, or the delta Opioid Receptor. CXCR2 plays an important role in attracting immune cells to sites of inflammation followed by their adhesion and extravasation. It is also involved in angiogenesis, the development of inflammatory disorders, and cancer. Rat CXCR2 shares 71% and 86% amino acid sequence identity with human and mouse CXCR2, respectively.

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Gene Symbol

CXCR2

UniProt

P35407

Additional CXCR2/IL-8RB Products

Product Documents for Rat CXCR2/IL-8RB Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat CXCR2/IL-8RB Antibody

For research use only

Citations for Rat CXCR2/IL-8RB Antibody

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