Rat CXCR2/IL-8RB PE-conjugated Antibody Summary
Accession # P35407
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CXCR2/IL‑8 RB in Rat Blood Granulocytes by Flow Cytometry. Rat peripheral blood granulocytes were stained with Mouse Anti-Rat CXCR2/IL-8 RB PE-conjugated Monoclonal Antibody (Catalog # FAB8110P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR2/IL‑8 RB in HEK Human Cell Line Transfected with Rat CSCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB PE-conjugated Monoclonal Antibody (Catalog # FAB8110P). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CXCR2 is an approximately 40 kDa 7-transmembrane domain receptor for the ELR+ chemokines CXCL1, 2, 3, 5, 6, 7, 8 and MIF. CXCR2 is expressed on neutrophils, monocytes, eosinophils, basophils, mast cells, T cells, oligodendrocytes, airway smooth muscle cells, and vascular endothelial cells. It is additionally upregulated in several cancers. CXCR2 can associate into homodimers or heterodimers with CXCR1, CXCR4, CD74, or the delta Opioid Receptor. CXCR2 plays an important role in attracting immune cells to sites of inflammation followed by their adhesion and extravasation. It is also involved in angiogenesis, the development of inflammatory disorders, and cancer. Rat CXCR2 shares 71% and 86% amino acid sequence identity with human and mouse CXCR2, respectively.
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