Rat Fas/TNFRSF6/CD95 Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Supplemental Products

Rat Fas/TNFRSF6/CD95 Biotinylated Antibody Summary

Species Reactivity
Detects rat Fas/TNFRSF6/CD95 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Fas, recombinant mouse Fas, and recombinant feline Fas (under non-reducing conditions) is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant rat Fas/TNFRSF6/CD95
Accession # NP_631933
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 2159-FA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, belongs to the death receptor family, which is a subfamily of the TNF receptor superfamily (1). Death receptors contain a cytoplasmic death domain (DD), which is required for transducing apoptotic signals. Engagement of Fas by its ligand (FasL) or agonistic anti-Fas antibodies induces dimerization and oligomerization of preformed Fas trimers. The activated receptor recruits the adaptor molecule FADD to form the Death-Inducing Signaling Complex (DISC) that also contains caspases. Upon activation, the caspases initiate a signaling cascade that induces the characteristic apoptotic phenotypes (2). Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. FasL is a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites (3).

Fas plays a role in the down-regulation of the immune reaction and has been shown to be an essential mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B-lymphocytes. Both human and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (4). Besides the perforin/granzyme-based mechanism, the Fas-FasL system has been identified as the alternate pathway for CTL-mediated cytotoxicity (5). FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells (6). Rat Fas cDNA encodes a 324 amino acid residue type 1 membrane protein. The extracellular domain of rat Fas shares 54.1% and 66.7% amino acid sequence identity with that of human and mouse Fas, respectively.

  1. Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
  2. Thorburn, S. (2003) Cellular Signaling 16:139.
  3. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA 94:5986.
  4. Siegel, R.M. et al. (2003) Immunol. Res. 27:499.
  5. Barry, M. et al. (2000) Mol. Cell Biol. 20:3781.
  6. Barreiro, R. et al. (2004) J. Immunol. 173:1519.
Long Name
Entrez Gene IDs
355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)
Alternate Names
Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Rat Fas/TNFRSF6/CD95 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Rat Fas/TNFRSF6/CD95 Biotinylated Antibody and earn rewards!

Have you used Rat Fas/TNFRSF6/CD95 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review