Rat FGF-BP Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human FGF-BP is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat FGF-BP
    Glu21-Cys238
    Accession # Q9QY10
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat FGF-BP (Catalog # 1413-FB)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Rat FGF-BP (Catalog # 1413-FB) to immobilized Recombinant Human FGF-basic (Catalog # 233-FB) coated at 5 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
FGF-BP in Rat Thymus. FGF-BP was detected in perfusion fixed frozen sections of rat thymus using Rat FGF-BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1413) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-BP

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2-4). FGF-BP contains a heparin-binding domain (aa 113-146) and a distinct FGF-binding region (aa 197-238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8-11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8-10, 12, 15, 16).

 

  • References:
    1. Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
    2. Aigner, A. et al. (2000) Growth Factors 18:51.
    3. Tassi, E. et al. (2006) Cancer Res. 66:1191.
    4. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
    5. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
    6. Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
    7. Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
    8. Beer, H-D. et al. (2005) Oncogene 24:5269.
    9. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
    10. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
    11. Kurtz, A. et al. (2004) Neoplasia 6:595.
    12. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
    13. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
    14. Kurtz, A. et al. (1997) Oncogene 14:2671.
    15. Aigner, A. et al. (2001) Int. J. Cancer 92:510.
    16. Czubayko, F. et al. (1997) Nat. Med. 3:1137.
  • Long Name:
    Fibroblast Growth Factor Binding Protein
  • Entrez Gene IDs:
    9982 (Human); 14181 (Mouse); 64535 (Rat)
  • Alternate Names:
    17 kDa heparin-binding growth factor-binding protein; FGFBP; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein
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